Stangeland and Salehian Clearing for GUS in Arabidopsis
نویسندگان
چکیده
Precise cellular localization of the GUS stain is notoriously difficult in Arabidopsis seeds. Here we report an improved protocol for the clearing of seeds after GUS staining. Incubation in ethanol–acetic acid (EtAc) and Hoyer’s medium allows reliable cellular localization of the GUS, even in seeds from late developmental stages. This method also leads to the staining of nucleoli in the endosperm and embryo, facilitating nuclear counts in endosperm development.
منابع مشابه
Molecular analysis of Arabidopsis endosperm and embryo promoter trap lines: reporter-gene expression can result from T-DNA insertions in antisense orientation, in introns and in intergenic regions, in addition to sense insertion at the 59 end of genes
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